Freelance Java Developer at Medutools Sp. z o.o., Kraków, Poland
Creating a java based web application, i.e. a search engine for medical products where the user can search for a medical product and get the list of medical doctors who can prescribe it, and the other way around, the user can type in a medical doctor and find which products the doctor can prescribe, »see project« , »see code«
Chief Product Officer at mReh Sp. z o.o., Kraków, Poland
Responsibilities: management of the mobiReh product, coordination with IT section, product development, writing and editing grant proposals (e.g. Horizon 2020, RPO), writing and editing scientific papers, implementation of the 3D model (which shows exercises for patients) into a desktop Java application, preparation of the user interface, and implementation of the event handling, »see project«
PhD candidate at the Jagiellonian University, Kraków, Poland
Project: Photoconversion of DNA-binding dyes and its application in super- resolution fluorescence microscopy. Supervisor: prof. Jerzy Dobrucki, contact: firstname.lastname@example.org, »link to references«, »link to PhD thesis«, »link to PhD defense presentation«, »see project«
PhD Visiting Student at IMDEA Nanociencia, Madrid, Spain (September-October)
Project: Visualisation of the DNA-binding dyes DAPI, Hoechst, or Vybrant DyeCycle Violet using single molecule localisation microscopy. Supervisor: prof. Cristina Flors, contact: email@example.com, »link to references«
PhD Visiting Student at the Institute of Molecular Biology, Mainz, Germany (January-February)
Project: Visualisation of the DNA-binding dye Vybrant DyeCycle Violet by means of single molecule localisation microscopy. Supervisor: prof. Christoph Cremer, contact: firstname.lastname@example.org, »link to references«
Masters student at the Jagiellonian University, Kraków, Poland
Project: Investigation of the recruitment process of the protein HP1 to the DNA damage site in a cell nucleus. Supervisor: prof. Jerzy Dobrucki, contact: email@example.com
Erasmus Student (July-October) at the Aarhus University, Aarhus, Denmark
Project: Studies of the plant plasma membrane proton pumps by X-ray crystallography to identify a regulatory K+ and Rb+ site and the dependence on different concentrations of various factors. Supervisor: prof. Poul Nissen, contact: firstname.lastname@example.org, »link to references«
Self-studying: Programming in Java
Java, JavaEE, SQL, HTML, CSS, Git, Maven, Bootstrap
PhD in Biophysics at the Jagiellonian University
Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology. Supervisor: prof. dr hab. Jerzy Dobrucki, contact: email@example.com »link do diploma«
Masters in Biophysics at the Jagiellonian University
Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology. Supervisor: prof. dr hab. Jerzy Dobrucki, contact: firstname.lastname@example.org, »link to diploma«
Awards and scholarships
Best Presentation at Life Science Open Space
mReh’s project mobiREH was awarded by Klaster Life Science at the Life Science Open Space conference.
Doctus Doctoral Scholarship
Scholarship awarded within the regional program of Małopolskie Centrum Przedsiębiorczości.
Project realised: Photoconversion of DNA-binding dyes DAPI and Hoechst as method for measuring the oxidative stress level in live cells. Project realised in collaboration with the biotechnological company BioCentrum sp. z o.o.
Poster Presentation, IMC2014: International Microscopy Congress, Prague
Title: UV-activated conversion of Hoechst and DAPI to their green-emitting protonated forms and their application in super-resolution microscopy.
Oral Presentation, II International Conference of Biophysics Students, Kraków
Title: UV-activated conversion of DNA dyes, Hoechst and DAPI, into blue-excited, green-to-orange emitting forms.
Oral Presentation, Poznań Days of Cytometry and Immunopathology, Poznań
Title: Photoconversion of the DNA dyes.
Poster Presentation, XL Jubilee Winter School of WBBiB, Zakopane
Title: UV-activated conversion of Hoechst 33258, DAPI and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms.
Oral Presentation, Workshop within the frames of Marie Curie Programme, Kraków
Title: Photoconversion of Hoechst and DAPI. Seventh Framework Programme (FP7/2007-2013), grant agreement no: 269156 (PIRSES-GA-2010-269156).
Oral Presentation, CYTO2012, Leipzig
Title: UV-activated conversion of DNA dyes, Hoechst and DAPI, into blue- excited, green-to-orange emitting forms. International Society for Advanced Cytometry.
Oral Presentation, Cytometry and Biokinetics Laboratory, Novosibirsk
Title: Histone methylation and heterochromatin protein 1 in DNA damage response. Institute of Chemical Kinetics and Combustion of SB RAS.
Gawrońska A, Żurek-Biesiada D, Rozkocha S, Golec J, Czechowska D, Masloń A. Two Years of Experience in Implementation of the mobiREH Remote Rehabilitation System Supporting Patients and Physiotherapists. Journal of the International Society for Telemedicine and EHealth, 2017, 5, (GKR);e23:(1-4) »link«
Żurek-Biesiada D, Szczurek AT, Prakash K, Best G, Mohana GK, Lee HK, Roignant JY, Dobrucki JW, Cremer C, Birk U. Quantitative super-resolution localization microscopy of DNA in situ using Vybrant® DyeCycle™ Violet fluorescent probe. Data Brief. 2016 Jan 29;7:157-71. doi: 10.1016/j.dib.2016.01.041. eCollection 2016 Jun. »link«
Żurek-Biesiada D, Szczurek A, Prakash K, Lee HK, Hagmann M, Dobrucki JW, Birk U, Cremer C. Localization microscopy of DNA in situ using Vybrant DyeCycle Violet fluorescent probe: A new approach to study nuclear nanostructure at single molecule resolution. Exp Cell Res. 2015 Sep 1. pii: S0014-4827(15)30080-X. doi: 10.1016/j.yexcr.2015.08.020 »link«
Szczurek A, Prakash K; Lee HK, Żurek-Biesiada D; Best G, Hagmann M, Dobrucki JW, Cremer C, Birk U. Single molecule localisation microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes. Nucleus. 2014; 19;5(4) »link«
Żurek-Biesiada D, Waligórski P, Dobrucki JW. UV-induced Spectral Shift and Protonation of DNA Fluorescent Dye Hoechst 33258. J Fluoresc. 2014 Nov;24(6):1791-801. doi: 10.1007/s10895-014-1468-y. Epub 2014 Oct 14. »link«
Żurek-Biesiada D, Kędracka-Krok S, Dobrucki JW. UV-activated conversion of Hoechst 33258, DAPI, and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms. Cytometry A. 2013 May;83(5):441-51. doi: 10.1002/cyto.a.22260. Epub 2013 Feb 15. »link«