Jan-Feb 2019 (Java)
Freelance Java Developer at Medutools Sp. z o.o., Kraków, Poland
Creating a search engine, where the user can choose a medical product and see which medical doctor can prescribe it, and the other way around, the user can search for a medical doctor and see which medical products they can prescribe. Application written in #java using #javaee framework, medical data stored in #json format, »see project« , »see the code«
Creating non-commercial projects in Java
1. 10 closest earthquakes – web application
An application in which the user types in the longitude and latitude of the desired city and as the results gets the list of 10 closest earthquakes that took place: »see the application«, »see the code«
2. Paint – desktop application
An drawing platform where the user can draw (lines, shapes), redo or undo all the actions, write text, load external images, save the existing drawings, or open new drawing panels of a preferred size: »download jar file«, »see the code«
2015-2019 (project management & Java)
Chief Product Officer at mReh Sp. z o.o., Kraków, Poland
Responsibilities: management of the mobiReh product, coordination with IT section, product development, writing and editing grant proposals (e.g. Horizon 2020, RPO), writing and editing scientific papers, implementation of the 3D model (which shows exercises for patients) into a desktop Java application, preparation of the user interface, and implementation of the event handling, »see the project«
Graduate Fellow – Biophysics, Jagiellonian University, Kraków, Poland
Doctoral research in the field of super-resolution fluorescence microscopy. Responsibilities: planning and conducting microscopy, mass spectromerty & spectrofluorimetry experiments, culturing cell lines, preparation of buffer solutions, analysis of scientific data, writing scientific grant applications, writing scientific papers, collaboration with other university units, problem solving, project management. Supervisor: prof. Jerzy Dobrucki, »references«, »thesis«, »defense presentation«, »see the project«
Research Intern at Cristina Flors Lab, IMDEA Nanociencia, Madrid, Spain (September-October)
Project: Visualisation of the DNA-binding dyes DAPI, Hoechst, or Vybrant DyeCycle Violet using single molecule localisation microscopy. Supervisor: prof. Cristina Flors, contact: email@example.com, »link to references«
Research Intern at Christoph Cremer Lab, Institute of Molecular Biology, Mainz, Germany (January-February)
Project: Visualisation of the DNA-binding dye Vybrant DyeCycle Violet by means of single molecule localisation microscopy. Supervisor: prof. Christoph Cremer, contact: firstname.lastname@example.org, »link to references«
Masters student at the Jagiellonian University, Kraków, Poland
Project: Investigation of the recruitment process of the protein HP1 to the DNA damage site in a cell nucleus. Supervisor: prof. Jerzy Dobrucki, contact: email@example.com
Erasmus Student (July-October) at the Aarhus University, Aarhus, Denmark
Project: Studies of the plant plasma membrane proton pumps by X-ray crystallography to identify a regulatory K+ and Rb+ site and the dependence on different concentrations of various factors. Supervisor: prof. Poul Nissen, contact: firstname.lastname@example.org, »link to references«
Self-studying: Programming in Java
Graduate Fellow – Biophysics, Jagiellonian University
Doctoral research at the Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology. Supervisor: prof. Jerzy Dobrucki, »link do diploma«
Awards and scholarships
Best Presentation at Life Science Open Space
mReh’s project mobiREH was awarded by Klaster Life Science at the Life Science Open Space conference.
Doctus Doctoral Scholarship
Scholarship awarded within the regional program of Małopolskie Centrum Przedsiębiorczości.
Project realised: Photoconversion of DNA-binding dyes DAPI and Hoechst as method for measuring the oxidative stress level in live cells. Project realised in collaboration with the biotechnological company BioCentrum sp. z o.o.
Poster Presentation, IMC2014: International Microscopy Congress, Prague
Title: UV-activated conversion of Hoechst and DAPI to their green-emitting protonated forms and their application in super-resolution microscopy.
Oral Presentation, II International Conference of Biophysics Students, Kraków
Title: UV-activated conversion of DNA dyes, Hoechst and DAPI, into blue-excited, green-to-orange emitting forms.
Oral Presentation, Poznań Days of Cytometry and Immunopathology, Poznań
Title: Photoconversion of the DNA dyes.
Poster Presentation, XL Jubilee Winter School of WBBiB, Zakopane
Title: UV-activated conversion of Hoechst 33258, DAPI and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms.
Oral Presentation, Workshop within the frames of Marie Curie Programme, Kraków
Title: Photoconversion of Hoechst and DAPI. Seventh Framework Programme (FP7/2007-2013), grant agreement no: 269156 (PIRSES-GA-2010-269156).
Oral Presentation, CYTO2012, Leipzig
Title: UV-activated conversion of DNA dyes, Hoechst and DAPI, into blue- excited, green-to-orange emitting forms. International Society for Advanced Cytometry.
Oral Presentation, Cytometry and Biokinetics Laboratory, Novosibirsk
Title: Histone methylation and heterochromatin protein 1 in DNA damage response. Institute of Chemical Kinetics and Combustion of SB RAS.
Gawrońska A, Żurek-Biesiada D, Rozkocha S, Golec J, Czechowska D, Masloń A. Two Years of Experience in Implementation of the mobiREH Remote Rehabilitation System Supporting Patients and Physiotherapists. Journal of the International Society for Telemedicine and EHealth, 2017, 5, (GKR);e23:(1-4) »link«
Żurek-Biesiada D, Szczurek AT, Prakash K, Best G, Mohana GK, Lee HK, Roignant JY, Dobrucki JW, Cremer C, Birk U. Quantitative super-resolution localization microscopy of DNA in situ using Vybrant® DyeCycle™ Violet fluorescent probe. Data Brief. 2016 Jan 29;7:157-71. doi: 10.1016/j.dib.2016.01.041. eCollection 2016 Jun. »link«
Żurek-Biesiada D, Szczurek A, Prakash K, Lee HK, Hagmann M, Dobrucki JW, Birk U, Cremer C. Localization microscopy of DNA in situ using Vybrant DyeCycle Violet fluorescent probe: A new approach to study nuclear nanostructure at single molecule resolution. Exp Cell Res. 2015 Sep 1. pii: S0014-4827(15)30080-X. doi: 10.1016/j.yexcr.2015.08.020 »link«
Szczurek A, Prakash K; Lee HK, Żurek-Biesiada D; Best G, Hagmann M, Dobrucki JW, Cremer C, Birk U. Single molecule localisation microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes. Nucleus. 2014; 19;5(4) »link«
Żurek-Biesiada D, Waligórski P, Dobrucki JW. UV-induced Spectral Shift and Protonation of DNA Fluorescent Dye Hoechst 33258. J Fluoresc. 2014 Nov;24(6):1791-801. doi: 10.1007/s10895-014-1468-y. Epub 2014 Oct 14. »link«
Żurek-Biesiada D, Kędracka-Krok S, Dobrucki JW. UV-activated conversion of Hoechst 33258, DAPI, and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms. Cytometry A. 2013 May;83(5):441-51. doi: 10.1002/cyto.a.22260. Epub 2013 Feb 15. »link«